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gdf9  (R&D Systems)


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    R&D Systems gdf9
    Gdf9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdf9/product/R&D Systems
    Average 93 stars, based on 12 article reviews
    gdf9 - by Bioz Stars, 2026-03
    93/100 stars

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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of <t>GDF9</t> in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation
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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of <t>GDF9</t> in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation
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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of <t>GDF9</t> in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation
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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of <t>GDF9</t> in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation
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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of <t>GDF9</t> in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation
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    Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of GDF9 in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation

    Journal: Cell communication and signaling : CCS

    Article Title: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation.

    doi: 10.1186/s12964-024-01827-z

    Figure Lengend Snippet: Fig. 5 Effect of AKG on cell-cell communication between cumulus and oocyte (A) The interaction weight plot of COCs. The thickness of the line indicates the number of interactions, and the stronger the interaction weight/intensity between the two cell types. Bar plot showed the total interaction weight of COCs in each group. (B) The up-regulated (increased) signaling ligand-receptor pairs were identified by comparing the communication probabilities in AKG30 samples and other samples. (C) Immunoblotting of GDF9 in control, 30 and 750 µM AKG treated oocytes. (D) The expression of GDF9 in oocytes, expressed using log2(TPM + 1), n = 8. (E) the fluorescence intensity of oocytes Calcein-AM to indicate the changes of Cumulus cell-oocyte gap junction permeability during oocyte maturation, n = 9. (F) Representative images of the fluorescence intensity of F-actin on the zona pellucida to indicate the changes of transzonal projections (TZPs) during oocyte maturation. (G) Line graph showed the changes of TZPs during oocyte maturation

    Article Snippet: In some experiments, the culture medium was supplemented with 100 ng/mL GDF9 protein (MCE, Shanghai, China) or 1 μM carbenoxolone disodium (CBX) (MCE, Shanghai, China).

    Techniques: Western Blot, Control, Expressing, Fluorescence, Permeability

    Fig. 6 Effects of AKG and GDF9 on cumulus cells expansion. (A) The expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus cells expressed by log2 (TPM + 1), n = 3. (B) Immunoblotting of HAS2 in control, 30 and 750 µM AKG treated cumulus cells. (C) Representative images of the COCs after 22 h of culture, Scale bar = 200 µM, n = 30. (D) Relative cumulus expansion area n = 30. (E) Cumulus expansion index, n = 30. (F) qPCR results showed the expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus, n = 3

    Journal: Cell communication and signaling : CCS

    Article Title: The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation.

    doi: 10.1186/s12964-024-01827-z

    Figure Lengend Snippet: Fig. 6 Effects of AKG and GDF9 on cumulus cells expansion. (A) The expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus cells expressed by log2 (TPM + 1), n = 3. (B) Immunoblotting of HAS2 in control, 30 and 750 µM AKG treated cumulus cells. (C) Representative images of the COCs after 22 h of culture, Scale bar = 200 µM, n = 30. (D) Relative cumulus expansion area n = 30. (E) Cumulus expansion index, n = 30. (F) qPCR results showed the expression of cumulus expansion related genes HAS2, PTX3, CD44 and HMMR in cumulus, n = 3

    Article Snippet: In some experiments, the culture medium was supplemented with 100 ng/mL GDF9 protein (MCE, Shanghai, China) or 1 μM carbenoxolone disodium (CBX) (MCE, Shanghai, China).

    Techniques: Expressing, Western Blot, Control